A full term female baby, with a birth weight of 2.24
kg presented with yellowish discoloration of eyes and skin at 18 hours
of life. Antenatally, there was no evidence of fetal anemia or hydrops
fetalis. On examination, baby had icterus till palms and soles but
mental status, cry and muscle tone were normal. Baby was started on
intensive phototherapy. Blood group was O positive in both the mother
and the baby. Laboratory investigations showed total serum bilirubin
(TSB) of 24 mg/dL with direct bilirubin (DB) of 2.72 mg/dL, reticulocyte
count of 24%, direct Coomb test (DCT) of 3+, and hemoglobin of 8.2 g/dL.
Peripheral smear was suggestive of hemolysis with macrocytes, microcytes,
spherocytes and polychromasia with nucleated RBC.
In view of strongly positive DCT and no Rh negative
or ABO setting and minor blood group incompatibility was done. The
results of antibody testing for screening tests revealed the presence of
multiple alloantibodies (anti-Rh17) in maternal serum. The Rh-kell
antigenic phenotyping of the family members were as follows: Mother
(blood group O, D+, C-, c-, E-, e-, K- (i.e D–), index case (blood group
O, D+, C+ c-, E-, e+, K-), father (D+, C+ c-, E-, e+, K-) and sibling
(D+, C+, c-, E+, e-, K+).
Baby was treated with intensive phototherapy and 1
g/kg of intravenous immunoglobulin (IVIG). Due to this rare variety of
blood group, cross matching was not compatible with the available blood
in various blood banks despite extensive search. By 30 hours of age,
serum bilirubin reduced to 13 mg/dL and was out of exchange transfusion
zone. Baby had rebound hyperbilirubinemia, probably due to mild
hemolysis by persisting antibodies, and required intensive phototherapy
on day 15 of life, and was discharged on day 19 of life. At discharge,
parents were advised for regular follow-up for jaundice and anemia, and
also counselled regarding the need of anti-Rh 17 antibody titers in next
pregnancy, and periodic monitoring for fetal anemia and possible need of
intrauterine transfusion.
The Rh blood group system is inherited as group of
Rh D and Rh CE genes located on chromosome 1 [1,2]. Based on
the expression of the major D antigen on their RBCs, typing as Rh
positive or negative is done. RBCs also express C or c, E or e antigens.
Rh deletion of C/c and E/e loci can rarely occur leading to D– blood
group phenotype [4].
The mother’s RBC Rh-kell phenotyping was (D–).
Individuals with D– phenotype produce multiple Rh alloantibodies known
as anti-Rh17 or Anti-Hro antibodies against C,c,E,e antigens if there is
a history of sensitization such as childbirth (fetomaternal hemorrhage)
or transfusion with red blood cells that express C/c or E/e antigens
[3]. Anti-Rh 17 antibody is an antibody against Hro (Rh17) antigen and
is produced in persons lacking any Rh group antigens except D antigen
[4].
The Rh-kell phenotype of the index case suggested
that the possible antibodies leading to hemolysis were anti-C and
anti-e. The management of anti Rh-17 isoimmunization is similar to the
management of Rh D isoimmunization including antenatal monitoring,
intrauterine transfusion, use of IVIG and exchange transfusion, with a
specification that blood used for fetal or neonatal transfusion should
be Rh17 antigen negative blood [3]. However, it may be extremely
difficult to get compatible or antigen negative red cells for
transfusion. A recent review of case reports showed that most of the
cases were managed by exchange transfusion, with or without intra
uterine transfusion, with very few cases managed by phototherapy alone
[5]. They also recommend the first choice of blood that needs to be used
in these neonates is washed and irradiated mothers’ RBCs. If this is not
available, then blood of relatives of pregnant woman, usually siblings,
can be used. Other options include storage of woman’s blood by freezing
the RBCs before pregnancy or use of blood from blood banks where this
blood is stored.
This case highlights the importance of considering
isoimmunization due to other Rh antigens as an important cause of
hemolytic disease of newborn.
1. Mina SS, Bhardwaj R, Gupta S. Hemolytic disease of
newborn: Can think beyond Rh (D) and ABO incompatibilities. J Clin
Neonat. 2017;6:37-39.
2. Landsteiner K, Wiener A. An agglutinable factor in
human blood recognized by immune sera for rhesus blood. Proc Soc Exp Bio
Med. 1940;43:223-223.
3. Choobdar AF, Milani H, Behrouzi K, et al.
Anti-Rh17 allo immunization: A rare case of severe hemolytic disease of
the newborn and review of the literature. J Pediatr Rev. 2020;8:29-34.
4. Mario. E, Oyen RR, Morsh WL. Summary of the
clinical significance of blood group alloantibodies. Sem Hematol.
2000;37:197-216.
5. Dajak S, Ipavec N, Cuk M, et al. The outcome of
hemolytic disease of the fetus and newborn caused by anti-rh17 antibody:
Analysis of three cases and review of the literature. Transfus Med
Hemother. 2020;47:264-71.